Anti-Human CD3 mAb

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Model: GMP-TL101

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Tags: Total Solution for T Cell Therapy , FDA DMF Filled Products

In immunology, CD3 (Differentiated Cluster 3) of T cells is a protein complex. CD3 has five peptide chains, namely, the γ, δ, ε, ζ, and η chains, all of which are transmembrane proteins. The transmembrane region of the CD3 molecule is connected to the transmembrane region of the two peptide chains of TCR through a salt bridge to form the TCR-CD3 complex, which jointly participates in the recognition of antigen. The activation signal generated by TCR antigen recognition is generated and transduced by CD3. Mononuclear cells isolated from peripheral blood, bone marrow, or umbilical cord blood can obtain large numbers of proliferating T lymphocytes in the presence of CD3 monoclonal antibodies and various cytokines.

Monoclonal Antibodies
Applications	Soluble method: the concentration of 500 ng/mL is recommended, under which T cells can be activated and CD3⁺CD56⁺T cells can be expanded significantly.
Biological activity	The binding rate with Jurkat cells shall not be less than 90%
Coating method:	10 mL PBS buffer (5μg/mL anti-human CD3) is placed in a T75 flask at 4°C overnight. Please remove the PBS and wash the flask with saline gently three times before use.
Endotoxin	< 0.01EU per μg of the protein as determined by the LAL method.
Expression Host	CHO cells
Formulation	Supplied as a 0.22μm filtered solution in PBS, PH 7.4
Purification：	Protein A purified from cell culture supernatant
QC Testing Purity	> 95 % as determined by SDS-PAGE
Stability & Storage	24 months at 2℃ to 8℃. Avoid repeated freeze-thaw cycles