Recombinant Human bFGF Protein

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Model: GMP-TL901

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Tags: MSC Cell Culture , iPSC related Products

Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated, heparin-binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumor growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.

Cytokines
Expression Host	E.coli
Synonym	FGF2, FGFB, FGF basic, HBGF-2
Protein Sequence	A DNA sequence encoding the human FGF2（P09038）was expressed with no tag.
Molecular Mass	Predicts a molecular mass of 17.1 kD.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per 1 μg of the protein by the LAL method.
Activity	Measured in a cell proliferation assay using Balb/c 3T3 cells. The ED₅₀ for this effect is ≤ 0.1ng/mL, corresponding to a specific activity of ≥ 1 x 10⁷IU/mg.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability	24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.