Recombinant Human EGF Protein
![Recombinant Human EGF Protein Recombinant Human EGF Protein](https://www.tlbiotechnology.com/image/cache/catalog/Cytokines-550x550w.png)
- Stock: In Stock
- Model: GMP-TL613
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EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation. EGF is widely used as a mitogen in the culture of neural stem cells in vitro, and it can be used as an inducer to promote the differentiation of neural stem cells into neurons, astrocytes and oligodendrocytes.
Cytokines | |
Expression Host | HEK293 cells |
Molecular Mass | Predicts a molecular mass of 7.0 kD. |
QC Testing Purity | > 90 % as determined by SDS-PAGE. |
Endotoxin | < 0.1EU per 1 μg of the protein by the LAL method. |
Activity | Measured in a cell proliferation assay using Balb/c 3T3 cells. The ED₅₀ for this effect is ≤3 ng/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization. |
Stability | 24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. |
Storage | Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100 μg/mL. Avoid repeated freeze-thaw cycles. |
Thumb | File information |
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GMP-TL613_SDS.pdf File Size161.29KB Downloaded: 80 |
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GMP-TL613_Product Sheet.pdf File Size81.18KB Downloaded: 79 |