Recombinant Human Fibronectin(FN) Protein
![Recombinant Human Fibronectin(FN) Protein Recombinant Human Fibronectin(FN) Protein](https://www.tlbiotechnology.com/image/cache/catalog/Cytokines-550x550w.png)
Recombinant Human Fibronectin(FN) Protein
- Stock: In Stock
- Model: GMP-TL903
$67.00
Available Options
Tags:
iPSC related Products
Recombinant Human Fibronectin(FN)Protein, including three functional regions (cell binding domain, heparin binding domain, and CS1 site), can significantly enhance retrovirus mediated gene transfer to mammalian cells. This effect may be due to colocalization of retroviral particles and target cells on rhFN CH-296 chimeric molecule via retroviral particles or heparin binding region II binding to cell adhesion sites VLA-4 and/or VLA-5. According to the research results of Hannenberg et al., there is typically 50% to 75% gene transduction of cells with the coating method.
Cytokines | |
Expression Host | E.coli |
Synonym | Cold-insoluble globulin |
Protein Sequence | A DNA sequence encoding the human Fibronectin(P02751)was expressed with a His-tag at the N-terminus. |
Molecular Mass | The Recombinant Human Fibronectin Protein predicts a molecular mass of 64.7 kD. |
QC Testing Purity | > 90 % as determined by SDS-PAGE |
Endotoxin | < 0.1EU per 1 μg of the protein as determined by the LAL method. |
Activity | Measured by its ability to support hiPS cell adhesion. The ED₅₀ for this effect is ≤ 3.2 μg/mL. |
Formulation | Supplied as a 0.22μm filtered solution in PBS, PH 7.4. |
Stability | Lyophilized preparation can be stored 24 months at -20℃. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. |
Storage | Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles. |
Thumb | File information |
![]() |
GMP-TL903_Product Sheet.pdf File Size93.42KB Downloaded: 59 |
![]() |
GMP-TL903_SDS.pdf File Size169.87KB Downloaded: 55 |