Recombinant Human Fibronectin(FN) Protein

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Model: GMP-TL903

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Recombinant Human Fibronectin(FN)Protein, including three functional regions (cell binding domain, heparin binding domain, and CS1 site), can significantly enhance retrovirus mediated gene transfer to mammalian cells. This effect may be due to colocalization of retroviral particles and target cells on rhFN CH-296 chimeric molecule via retroviral particles or heparin binding region II binding to cell adhesion sites VLA-4 and/or VLA-5. According to the research results of Hannenberg et al., there is typically 50% to 75% gene transduction of cells with the coating method.

Cytokines
Expression Host	E.coli
Synonym	Cold-insoluble globulin
Protein Sequence	A DNA sequence encoding the human Fibronectin(P02751)was expressed with a His-tag at the N-terminus.
Molecular Mass	The Recombinant Human Fibronectin Protein predicts a molecular mass of 64.7 kD.
QC Testing Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 0.1EU per 1 μg of the protein as determined by the LAL method.
Activity	Measured by its ability to support hiPS cell adhesion. The ED₅₀ for this effect is ≤ 3.2 μg/mL.
Formulation	Supplied as a 0.22μm filtered solution in PBS, PH 7.4.
Stability	Lyophilized preparation can be stored 24 months at -20℃. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.