GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 (cellular) immune responses in cognate T cells. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has been utilized in the clinical management of multiple disease processes.
Cytokines
Expression Host
HEK293 cells
Synonym
GM-CSF,CSF2,MGC131935
Protein Sequence
NP_000749.2：A18-E144 was expressed with a His-tag at the C-terminus.
Molecular Mass
Predicts a molecular mass of 15.3kD.
QC Testing Purity
> 90 % as determined by SDS-PAGE.
Endotoxin
< 0.1EU per 1 μg of the protein by the LAL method.
Activity
Measured by the dose-dependent stimulation of the proliferation of TF-1 cells，the specific activity is ≥5.0×10⁶IU/mg.
Formulation
Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability
Lyophilized preparation can be stored at -20 ℃.
6 months at -20℃ under sterile conditions after reconstitution.
12 months at -80℃ under sterile conditions after reconstitution.
Storage
Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL.
Avoid repeated freeze-thaw cycles.
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