Recombinant Human PDGF-BB Protein

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Model: GMP-TL644

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Tags: MSC Cell Culture

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules, and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors of PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is a high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts only with PDGF-BB and PDGF-AB.

Cytokines
Expression Host	HEK293 cells
Synonym	PDGFBB(Platelet-Derived Growth Factor-BB), PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis Glioma-derived growth factor(GDGF), Osteosarcoma-derived Growth Factor(ODGF)
Protein Sequence	A DNA sequence encoding the human PDGF-BB (GenBank: CAA45383.1) was expressed with a polyhistidine tag at the C-terminus.
Molecular Mass	The Recombinant Human PDGF-BB consists of 115 amino acids and predicts a molecular mass of 13.1 kDa.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per μg of the protein as determined by the LAL method.
Activity	Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED₅₀ for this effect is ≤100ng/mL.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6% mannitol are added as protectants before lyophilization.
Stability	Lyophilized preparation can be stored at -20 ℃. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.