Frequently Asked Questions
Both methods are feasible.
There will be no problem within a week.
PBMCs that have been cryopreserved and revived can be used to culture NK cells, but the culture effect is not as good as fresh PBMCs.
DPBS or serum-free culture medium can be used. It is not recommended to contain calcium and magnesium ions in the buffer system.
unit/mg=1000000/ED50(ng/ml)
Purity is defined as the proportion of collected target cells and can be analyzed by flow cytometry. The seperation efficiency is defined as the proportion of recovered target cells relative to the target cells in the original sample.
The purity varies depending on the cell viability/status/T cell ratio of the sample.
Customers conduct virus transfection experiments at 24h,48h or 72h, with 48h being the most common. When the virus is transfected, magnetic beads can be retained.
Not recommended for reuse.
It suggests removing the magnetic beads before testing the cells if they cannot.
Do GMP-TL603 magnetic beads need to be removed when collecting cells after activation and expansion?
Magnetic beads must be removed, general magnetic beads and magnetic frames can meet the application.
50nm. Magnetic beads need to go through separation columns (It is compatible with Miltenyi MACS columns), which are biodegradable.
The naked beads of micrometer magnetic beads (GMP-TL603) and nano magnetic beads (TL-622, TL-623, TL-624) have completed safety evaluation and verification at the animal level.